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1xbr
From Proteopedia
| 1xbr, resolution 2.50Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | XBRA (Xenopus laevis) | ||||||||
| Domains: | TBOX | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
T DOMAIN FROM XENOPUS LAEVIS BOUND TO DNA
The mouse Brachyury (T) gene is the prototype of a growing family of so-called T-box genes which encode transcriptional regulators and have been identified in a variety of invertebrates and vertebrates, including humans. Mutations in Brachyury and other T-box genes result in drastic embryonic phenotypes, indicating that T-box gene products are essential in tissue specification, morphogenesis and organogenesis. The T-box encodes a DNA-binding domain of about 180 amino-acid residues, the T domain. Here we report the X-ray structure of the T domain from Xenopus laevis in complex with a 24-nucleotide palindromic DNA duplex. We show that the protein is bound as a dimer, interacting with the major and the minor grooves of the DNA. A new type of specific DNA contact is seen, in which a carboxy-terminal helix is deeply embedded into an enlarged minor groove without bending the DNA. Hydrophobic interactions and an unusual main-chain carbonyl contact to a guanine account for sequence-specific recognition in the minor groove by this helix. Thus the structure of this T domain complex with DNA reveals a new way in which a protein can recognize DNA.
Crystallographic structure of the T domain-DNA complex of the Brachyury transcription factor., Muller CW, Herrmann BG, Nature. 1997 Oct 23;389(6653):884-8. PMID:9349824
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1XBR is a 4 chains structure with sequences from Xenopus laevis. Full crystallographic information is available from OCA.
See also
- Brachyury transcription factor
Reference
- Muller CW, Herrmann BG. Crystallographic structure of the T domain-DNA complex of the Brachyury transcription factor. Nature. 1997 Oct 23;389(6653):884-8. PMID:9349824 doi:10.1038/39929
Page seeded by OCA on Wed Jul 7 14:41:50 2010
