CRYSTAL STRUCTURE OF RAT TOM20-ALDH PRESEQUENCE COMPLEX
[[2v1t]], [[Resolution|resolution]] 1.92Å
2v1t is a 4 chain structure with sequence from Rattus norvegicus. This structure supersedes the now removed PDB entry 1wt4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Most mitochondrial proteins are synthesized in the cytosol and imported into mitochondria. The N-terminal presequences of mitochondrial-precursor proteins contain a diverse consensus motif (phi chi chi phi phi, phi is hydrophobic and chi is any amino acid), which is recognized by the Tom20 protein on the mitochondrial surface. To reveal the structural basis of the broad selectivity of Tom20, the Tom20-presequence complex was crystallized. Tethering a presequence peptide to Tom20 through a disulfide bond was essential for crystallization. Unexpectedly, the two crystals with different linker designs provided unique relative orientations of the presequence with respect to Tom20, and neither configuration could fully account for the hydrophobic preference at the three hydrophobic positions of the consensus motif. We propose the existence of a dynamic equilibrium in solution among multiple states including the two bound states. In accordance, NMR 15N relaxation analyses suggested motion on a sub-millisecond timescale at the Tom20-presequence interface. We suggest that the dynamic, multiple-mode interaction is the molecular mechanism facilitating the broadly selective specificity of the Tom20 receptor toward diverse mitochondrial presequences.
Tom20 recognizes mitochondrial presequences through dynamic equilibrium among multiple bound states.,Saitoh T, Igura M, Obita T, Ose T, Kojima R, Maenaka K, Endo T, Kohda D EMBO J. 2007 Nov 14;26(22):4777-87. Epub 2007 Oct 18. PMID:17948058
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Saitoh T, Igura M, Obita T, Ose T, Kojima R, Maenaka K, Endo T, Kohda D. Tom20 recognizes mitochondrial presequences through dynamic equilibrium among multiple bound states. EMBO J. 2007 Nov 14;26(22):4777-87. Epub 2007 Oct 18. PMID:17948058 doi:7601888