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1vyb
From Proteopedia
| 1vyb, resolution 1.80Å () | |||||||||
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| Sites: | |||||||||
| Ligands: | , , | ||||||||
| Domains: | Exo_endo_phos | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
ENDONUCLEASE DOMAIN OF HUMAN LINE1 ORF2P
The human L1 endonuclease (L1-EN) is encoded by the non-LTR retrotransposon LINE-1 (L1). L1 is responsible for more than 1.5 million retrotransposition events in the history of the human genome, contributing more than a quarter to human genomic DNA (L1 and Alu elements). L1-EN is related to the well-understood human DNA repair endonuclease APE1, and its nicking specificity is a major determinant for retrotransposon integration site selection. The crystal structure of human L1 endonuclease is the first of a retrotransposon-encoded protein and a prototype for retrotransposon-encoded endonucleases involved in target-primed reverse transcription. Structure-based endonuclease alignments reveal a conserved threonine in addition to previously identified invariant residues and suggest that DNA recognition proceeds via the accommodation of an extrahelical nucleotide within a pocket of the enzyme. The present analysis will help to refine phylogenetic and functional relationships among metal-dependent phosphohydrolases and provides a basis for manipulating non-LTR retrotransposon integration site selection.
Crystal structure of the targeting endonuclease of the human LINE-1 retrotransposon., Weichenrieder O, Repanas K, Perrakis A, Structure. 2004 Jun;12(6):975-86. PMID:15274918
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1VYB is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Weichenrieder O, Repanas K, Perrakis A. Crystal structure of the targeting endonuclease of the human LINE-1 retrotransposon. Structure. 2004 Jun;12(6):975-86. PMID:15274918 doi:10.1016/j.str.2004.04.011
Page seeded by OCA on Mon Feb 16 17:56:46 2009
