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1uw0
From Proteopedia
| 1uw0, 28 NMR models () | |||||||||
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| Ligands: | |||||||||
| Activity: | DNA ligase (ATP), with EC number 6.5.1.1 | ||||||||
| Domains: | zf-PARP | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
SOLUTION STRUCTURE OF THE ZINC-FINGER DOMAIN FROM DNA LIGASE IIIA
DNA ligase IIIalpha carries out the final ligation step in the base excision repair (BER) and single strand break repair (SSBR) mechanisms of DNA repair. The enzyme recognises single-strand nicks and other damage features in double-stranded DNA, both through the catalytic domain and an N-terminal domain containing a single zinc finger. The latter is homologous to other zinc fingers that recognise damaged DNA, two in the N terminus of poly(adenosine-ribose)polymerase and three in the N terminus of the Arabidopsis thaliana nick-sensing DNA 3'-phosphoesterase. Here, we present the solution structure of the zinc-finger domain of human DNA ligase IIIalpha, the first structure of a finger from this group. It is related to that of the erythroid transcription factor GATA-1, but has an additional N-terminal beta-strand and C-terminal alpha-helix. Chemical shift mapping using a DNA ligand containing a single-stranded break showed that the DNA-binding surface of the DNA-ligase IIIalpha zinc finger is substantially different from that of GATA-1, consistent with the fact that the two proteins recognise very different features in the DNA. Likely implications for DNA binding are discussed.
Solution structure and DNA binding of the zinc-finger domain from DNA ligase IIIalpha., Kulczyk AW, Yang JC, Neuhaus D, J Mol Biol. 2004 Aug 13;341(3):723-38. PMID:15288782
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1UW0 is a 1 chain structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
- Kulczyk AW, Yang JC, Neuhaus D. Solution structure and DNA binding of the zinc-finger domain from DNA ligase IIIalpha. J Mol Biol. 2004 Aug 13;341(3):723-38. PMID:15288782 doi:10.1016/j.jmb.2004.06.035
Page seeded by OCA on Tue Feb 17 18:10:46 2009
