First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
1ulh
From Proteopedia
| 1ulh, resolution 2.31Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | trps (Homo sapiens) | ||||||||
| Activity: | Tryptophan--tRNA ligase, with EC number 6.1.1.2 | ||||||||
| Domains: | TrpRS_core | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase
Human tryptophanyl-tRNA synthetase (TrpRS) is secreted into the extracellular region of vascular endothelial cells. The splice variant form (mini TrpRS) functions in vascular endothelial cell apoptosis as an angiostatic cytokine. In contrast, the closely related human tyrosyl-tRNA synthetase (TyrRS) functions as an angiogenic cytokine in its truncated form (mini TyrRS). Here, we determined the crystal structure of human mini TrpRS at a resolution of 2.3 A and compared the structure with those of prokaryotic TrpRS and human mini TyrRS. Deletion of the tRNA anticodon-binding (TAB) domain insertion, consisting of eight residues in the human TrpRS, abolished the enzyme's apoptotic activity for endothelial cells, whereas its translational catalysis and cell-binding activities remained unchanged. Thus, we have identified the inserted peptide motif that activates the angiostatic signaling.
A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase., Kise Y, Lee SW, Park SG, Fukai S, Sengoku T, Ishii R, Yokoyama S, Kim S, Nureki O, Nat Struct Mol Biol. 2004 Feb;11(2):149-56. Epub 2004 Jan 11. PMID:14730354
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1ULH is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Kise Y, Lee SW, Park SG, Fukai S, Sengoku T, Ishii R, Yokoyama S, Kim S, Nureki O. A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase. Nat Struct Mol Biol. 2004 Feb;11(2):149-56. Epub 2004 Jan 11. PMID:14730354 doi:10.1038/nsmb722
Page seeded by OCA on Mon Feb 16 14:29:23 2009
Categories: Homo sapiens | Tryptophan--tRNA ligase | Ishii, R. | Kim, S. | Kise, Y. | Lee, S W. | Nureki, O. | Park, S G. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Sengoku, T. | Yokoyama, S. | Aminoacylation | Angiostatic cytokine | Apoptosis | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
