1uko

From Proteopedia

Jump to: navigation, search


1uko, resolution 2.10Å ()
Ligands:
Activity: Beta-amylase, with EC number 3.2.1.2
Related: 1bya, 1byb, 1byc, 1byd, 1bfn, 1fa2, 1ukp
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal structure of soybean beta-amylase mutant substituted at surface region

Publication Abstract from PubMed

In spite of the high similarity of amino acid sequence and three-dimensional structure between soybean beta-amylase (SBA) and sweet potato beta-amylase (SPB), their quaternary structure is quite different, being a monomer in SBA and a tetramer in SPB. Because most of the differences in amino acid sequences are found in the surface region, we tested the tetramerization of SBA by examining mutations of residues located at the surface. We designed the SBA tetramer using the SPB tetramer structure as a model and calculating the change of accessible surface area (DeltaASA) for each residue in order to select sites for the mutation. Two different mutant genes encoding SB3 (D374Y/L481R/P487D) and SB4 (K462S added to SB3), were constructed for expression in Escherichia coli and the recombinant proteins were purified. They existed as a monomer in solution, but gave completely different crystals from the native SBA. The asymmetric unit of the mutants contains four molecules, while that of native SBA contains one. The interactions of the created interfaces revealed that there were more intermolecular interactions in the SB3 than in the SB4 tetramer. The substituted charged residues on the surface are involved in interactions with adjacent molecules in a different way, forming a new crystal packing pattern.

Change in the crystal packing of soybean beta-amylase mutants substituted at a few surface amino acid residues., Kang YN, Adachi M, Mikami B, Utsumi S, Protein Eng. 2003 Nov;16(11):809-17. PMID:14631070

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1uko is a 4 chain structure with sequence from Glycine max. Full crystallographic information is available from OCA.

See Also

Reference

  • Kang YN, Adachi M, Mikami B, Utsumi S. Change in the crystal packing of soybean beta-amylase mutants substituted at a few surface amino acid residues. Protein Eng. 2003 Nov;16(11):809-17. PMID:14631070

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools