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1toz
From Proteopedia
| 1toz, 20 NMR models () | |||||||||
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| Related: | 1emo, 1lmj, 1hj7 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
NMR structure of the human NOTCH-1 ligand binding region
We present NMR structural and dynamics analysis of the putative ligand binding region of human Notch-1, comprising EGF-like domains 11-13. Functional integrity of an unglycosylated, recombinant fragment was confirmed by calcium-dependent binding of tetrameric complexes to ligand-expressing cells. EGF modules 11 and 12 adopt a well-defined, rod-like orientation rigidified by calcium. The interdomain tilt is similar to that found in previously studied calcium binding EGF pairs, but the angle of twist is significantly different. This leads to an extended double-stranded beta sheet structure, spanning the two EGF modules. Based on the conservation of residues involved in interdomain hydrophobic packing, we propose this arrangement to be prototypical of a distinct class of EGF linkages. On this premise, we have constructed a model of the 36 EGF modules of the Notch extracellular domain that enables predictions to be made about the general role of calcium binding to this region.
Structural and functional properties of the human notch-1 ligand binding region., Hambleton S, Valeyev NV, Muranyi A, Knott V, Werner JM, McMichael AJ, Handford PA, Downing AK, Structure. 2004 Dec;12(12):2173-83. PMID:15576031
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1toz is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
- Hambleton S, Valeyev NV, Muranyi A, Knott V, Werner JM, McMichael AJ, Handford PA, Downing AK. Structural and functional properties of the human notch-1 ligand binding region. Structure. 2004 Dec;12(12):2173-83. PMID:15576031 doi:10.1016/j.str.2004.09.012
- Muranyi A, Hambleton S, Knott V, McMichael A, Handford PA, Downing AK. 1H, 13C, and 15N resonance assignments of human Notch-1 calcium binding EGF domains 11-13. J Biomol NMR. 2004 Jul;29(3):443-4. PMID:15213460 doi:10.1023/B:JNMR.0000032521.42723.1a
