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1s1e
From Proteopedia
| 1s1e, resolution 2.30Å () | |||||||||
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| Ligands: | |||||||||
| Gene: | KCNIP1 (Homo sapiens) | ||||||||
| Domains: | EFh, FRQ1 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal Structure of Kv Channel-interacting protein 1 (KChIP-1)
The family of calcium binding proteins called KChIPs associates with Kv4 family K(+) channels and modulates their biophysical properties. Here, using mutagenesis and X-ray crystallography, we explore the interaction between Kv4 subunits and KChIP1. Two regions in the Kv4.2 N terminus, residues 7-11 and 71-90, are necessary for KChIP1 modulation and interaction with Kv4.2. When inserted into the Kv1.2 N terminus, residues 71-90 of Kv4.2 are also sufficient to confer association with KChIP1. To provide a structural framework for these data, we solved the crystal structures of Kv4.3N and KChIP1 individually. Taken together with the mutagenesis data, the individual structures suggest that that the Kv4 N terminus is required for stable association with KChIP1, perhaps through a hydrophobic surface interaction, and that residues 71-90 in Kv4 subunits form a contact loop that mediates the specific association of KChIPs with Kv4 subunits.
Two N-terminal domains of Kv4 K(+) channels regulate binding to and modulation by KChIP1., Scannevin RH, Wang K, Jow F, Megules J, Kopsco DC, Edris W, Carroll KC, Lu Q, Xu W, Xu Z, Katz AH, Olland S, Lin L, Taylor M, Stahl M, Malakian K, Somers W, Mosyak L, Bowlby MR, Chanda P, Rhodes KJ, Neuron. 2004 Feb 19;41(4):587-98. PMID:14980207
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1S1E is a 1 chain structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Scannevin RH, Wang K, Jow F, Megules J, Kopsco DC, Edris W, Carroll KC, Lu Q, Xu W, Xu Z, Katz AH, Olland S, Lin L, Taylor M, Stahl M, Malakian K, Somers W, Mosyak L, Bowlby MR, Chanda P, Rhodes KJ. Two N-terminal domains of Kv4 K(+) channels regulate binding to and modulation by KChIP1. Neuron. 2004 Feb 19;41(4):587-98. PMID:14980207
Page seeded by OCA on Wed Feb 18 02:01:45 2009
Categories: Homo sapiens | Bowlby, M R. | Carroll, K C. | Chanda, P. | Edris, W. | Jow, F. | Katz, A H. | Kopsco, D C. | Lin, L. | Lu, Q. | Malakian, K. | Megules, J. | Mosyak, L. | Olland, S. | Rhodes, K J. | Scannevin, R H. | Somers, W. | Stahl, M. | Taylor, M. | Wang, K W. | Xu, W X. | Xu, Z B. | Calcium-binding protein | Ef-finger | Kchip | Kv channel-interacting protein
