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1s0v
From Proteopedia
| 1s0v, resolution 3.20Å () | |||||||||
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| Ligands: | , | ||||||||
| Gene: | 1 (Enterobacteria phage T7) | ||||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||||
| Related: | 1h38 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Structural basis for substrate selection by T7 RNA polymerase
The mechanism by which nucleotide polymerases select the correct substrate is of fundamental importance to the fidelity of DNA replication and transcription. During the nucleotide addition cycle, pol I DNA polymerases undergo the transition from a catalytically inactive "open" to an active "closed" conformation. All known determinants of substrate selection are associated with the "closed" state. To elucidate if this mechanism is conserved in homologous single subunit RNA polymerases (RNAPs), we have determined the structure of T7 RNAP elongation complex with the incoming substrate analog. Surprisingly, the substrate specifically binds to RNAP in the "open" conformation, where it is base paired with the acceptor template base, while Tyr639 provides discrimination of ribose versus deoxyribose substrates. The structure therefore suggests a novel mechanism, in which the substrate selection occurs prior to the isomerization to the catalytically active conformation. Modeling of multisubunit RNAPs suggests that this mechanism might be universal for all RNAPs.
Structural basis for substrate selection by t7 RNA polymerase., Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG, Cell. 2004 Feb 6;116(3):381-91. PMID:15016373
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1s0v is a 16 chain structure with sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.
See Also
Reference
- Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG. Structural basis for substrate selection by t7 RNA polymerase. Cell. 2004 Feb 6;116(3):381-91. PMID:15016373
Categories: DNA-directed RNA polymerase | Enterobacteria phage t7 | Anikin, M. | McAllister, W T. | Patlan, V. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Temiakov, D. | Vassylyev, D G. | Yokoyama, S. | Dna | Riken structural genomics/proteomics initiative | Rna | Rsgi | Structural genomic | T7 rna polymerase | Transferase-dna-rna complex | Transferase-dna-rna hybrid complex
