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1ryk
From Proteopedia
| 1ryk, 20 NMR models () | |||||
|---|---|---|---|---|---|
| Gene: | YJBJ, B4045, Z5644, ECS5028, SF4160, S3571 (Escherichia coli) | ||||
| Domains: | PRK10428 | ||||
| Related: | 1jyg | ||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||
| Coordinates: | save as pdb, mmCIF, xml | ||||
Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;
The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.
An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1RYK is a 1 chain structure of sequence from Escherichia coli. Full experimental information is available from OCA.
Reference
An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485
Page seeded by OCA on Sun Nov 16 17:40:50 2008
Categories: Escherichia coli | Arrowsmith, C H. | Cort, J R. | Edwards, A M. | Kennedy, M A. | Liao, J. | NESG, Northeast Structural Genomics Consortium. | Pineda-Lucena, A. | Wu, B. | Yee, A. | Alpha protein | Nesg | Northeast structural genomics consortium | Ocsp | Protein structure initiative | Psi | Structural genomic
