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1rpu
From Proteopedia
| 1rpu, resolution 2.50Å () | |||||||||
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| Gene: | p19 (Carnation Italian ringspot virus) | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal Structure of CIRV p19 bound to siRNA
RNA silencing in plants likely exists as a defense mechanism against molecular parasites such as RNA viruses, retrotransposons, and transgenes. As a result, many plant viruses have adapted mechanisms to evade and suppress gene silencing. Tombusviruses express a 19 kDa protein (p19), which has been shown to suppress RNA silencing in vivo and bind silencing-generated and synthetic small interfering RNAs (siRNAs) in vitro. Here we report the 2.5 A crystal structure of p19 from the Carnation Italian ringspot virus (CIRV) bound to a 21 nt siRNA and demonstrate in biochemical and in vivo assays that CIRV p19 protein acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.
Size selective recognition of siRNA by an RNA silencing suppressor., Vargason JM, Szittya G, Burgyan J, Tanaka Hall TM, Cell. 2003 Dec 26;115(7):799-811. PMID:14697199
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1RPU is a 4 chains structure of sequences from Carnation italian ringspot virus. Full crystallographic information is available from OCA.
This structure is used in an entry detailing the structural basis of Plant Viral Protein p19 suppression of RNA Silencing and can be found here.
This structure is covered under the topic of Suppression of RNA Silencing by Viruses.
Reference
- Vargason JM, Szittya G, Burgyan J, Tanaka Hall TM. Size selective recognition of siRNA by an RNA silencing suppressor. Cell. 2003 Dec 26;115(7):799-811. PMID:14697199
Original page seeded by OCA on Tue Feb 17 21:38:22 2009
Categories: RNA Silencing | Post-transcriptional gene silencing (PTGS) | Virus | Plant Virus | Double-stranded RNA (dsRNA) | SiRNA | Small Interfering RNA | Carnation italian ringspot virus | Burgyan, J. | Hall, T M.T. | Szittya, G. | Vargason, J M. | Protein-rna complex | Protein complex | Rna double helix | A-form helix | Rna length recognition | Rnai
