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1rj7
From Proteopedia
| 1rj7, resolution 2.30Å () | |||||||||
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| Gene: | ED1, EDA (Homo sapiens) | ||||||||
| Related: | 1rj8 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of EDA-A1
EDA is a tumor necrosis factor family member involved in ectodermal development. Splice variants EDA-A1 and EDA-A2 differ only by the presence of Glu 308 and Val 309 in the expected receptor binding region of EDA-A1 but not EDA-A2. This two amino acid difference functions as a switch controlling receptor specificity. EDA-A1 binds only to EDAR, while EDA-A2 is specific for XEDAR. In order to understand the structural basis of this switch, we determined the X-ray crystal structures of the TNF domain of both EDA-A1 and EDA-A2 at 2.3 A and 2.2 A, respectively. While the backbone conformation around the splice difference is similar in both isoforms, the conformation of the following loop, the surface charge, and the shape of the expected receptor binding site differ significantly.
The crystal structures of EDA-A1 and EDA-A2: splice variants with distinct receptor specificity., Hymowitz SG, Compaan DM, Yan M, Wallweber HJ, Dixit VM, Starovasnik MA, de Vos AM, Structure. 2003 Dec;11(12):1513-20. PMID:14656435
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1rj7 is a 12 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Hymowitz SG, Compaan DM, Yan M, Wallweber HJ, Dixit VM, Starovasnik MA, de Vos AM. The crystal structures of EDA-A1 and EDA-A2: splice variants with distinct receptor specificity. Structure. 2003 Dec;11(12):1513-20. PMID:14656435
- Laskowski RA, Thornton JM. Understanding the molecular machinery of genetics through 3D structures. Nat Rev Genet. 2008 Feb;9(2):141-51. PMID:18160966 doi:10.1038/nrg2273
