First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
|1q8v, resolution 1.85Å ()|
|Related:||1n3o, 1n3p, 1n3q, 1ukg, 1q8o, 1q8p, 1q8q, 1q8s|
Pterocarpus angolensis lectin (PAL) in complex with the trimannoside [Man(Alpha1-3)]Man(alpha1-6)Man
The crystal structure of a Man/Glc-specific lectin from the seeds of the bloodwood tree (Pterocarpus angolensis), a leguminous plant from central Africa, has been determined in complex with mannose and five manno-oligosaccharides. The lectin contains a classical mannose-specificity loop, but its metal-binding loop resembles that of lectins of unrelated specificity from Ulex europaeus and Maackia amurensis. As a consequence, the interactions with mannose in the primary binding site are conserved, but details of carbohydrate-binding outside the primary binding site differ from those seen in the equivalent carbohydrate complexes of concanavalin A. These observations explain the differences in their respective fine specificity profiles for oligomannoses. While Man(alpha1-3)Man and Man(alpha1-3)[Man(alpha1-6)]Man bind to PAL in low-energy conformations identical with that of ConA, Man(alpha1-6)Man is required to adopt a different conformation. Man(alpha1-2)Man can bind only in a single binding mode, in sharp contrast to ConA, which creates a higher affinity for this disaccharide by allowing two binding modes.
Structural basis of oligomannose recognition by the Pterocarpus angolensis seed lectin., Loris R, Van Walle I, De Greve H, Beeckmans S, Deboeck F, Wyns L, Bouckaert J, J Mol Biol. 2004 Jan 30;335(5):1227-40. PMID:14729339
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Loris R, Van Walle I, De Greve H, Beeckmans S, Deboeck F, Wyns L, Bouckaert J. Structural basis of oligomannose recognition by the Pterocarpus angolensis seed lectin. J Mol Biol. 2004 Jan 30;335(5):1227-40. PMID:14729339