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1pp5
From Proteopedia
| 1pp5, 10 NMR models () | |||||||
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| Gene: | MCJA (Escherichia coli) | ||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Antibacterial Peptide Microcin J25: a 21-Residue Lariat Protoknot
The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.
Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot., Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH, J Am Chem Soc. 2003 Oct 15;125(41):12382-3. PMID:14531661
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1PP5 is a 1 chain structure of sequence from Escherichia coli. Full experimental information is available from OCA.
Reference
Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot., Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH, J Am Chem Soc. 2003 Oct 15;125(41):12382-3. PMID:14531661
Page seeded by OCA on Sun Nov 16 17:57:53 2008
Categories: Escherichia coli | Bayro, M J. | Ebright, R H. | Huang, J Y. | Ma, L C. | Montelione, G T. | Mukhopadhyay, J. | NESG, Northeast Structural Genomics Consortium. | Swapna, G V.T. | Backbone-sidechain amide linkage | Lariat | Nesg | Northeast structural genomics consortium | Protein structure initiative | Protoknot | Psi | Structural genomic
