1p15

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1p15, resolution 2.00Å ()
Gene: PTPRA OR LRP OR PTPA (Mus musculus)
Activity: Protein-tyrosine-phosphatase, with EC number 3.1.3.48
Related: 1p13
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal structure of the D2 domain of RPTPa

Publication Abstract from PubMed

The receptor protein tyrosine phosphatase alpha (RPTPalpha) is a transmembrane receptor with two intracellular protein tyrosine phosphatase domains, a catalytically active membrane proximal domain (D1) and a membrane distal phosphatase domain with minimal catalytic activity (D2). Here we elucidate the crystal structure of RPTPalpha's D2 domain. Unlike D1, D2 exists as a monomer and lacks the N-terminal inhibitory wedge motif. The N-terminal portion of D2 is disordered, and this region linking D1 to D2 is proteolytically labile in solution whether part of D2 alone or tethered to D1, indicating that the polypeptide backbone of this part of D2 is highly flexible, and therefore accessible to proteases under native conditions. Furthermore, we have crystallized the SH2 domain of the protein tyrosine kinase c-Src, a RPTPalpha substrate, with a phosphopeptide encompassing the C-terminal phosphorylation site of D2 (pTyr789). The SH2 domain of Src binds RPTPalpha in an extended conformation. The structural and functional data support a D1-D2 arrangement with significant flexibility between phosphatase domains of RPTPalpha that is likely to be important for dynamic alterations in intra- and/or intermolecular interactions that are critical for RPTPalpha function.

The structure of the membrane distal phosphatase domain of RPTPalpha reveals interdomain flexibility and an SH2 domain interaction region., Sonnenburg ED, Bilwes A, Hunter T, Noel JP, Biochemistry. 2003 Jul 8;42(26):7904-14. PMID:12834342

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1p15 is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA.

See Also

Reference

  • Sonnenburg ED, Bilwes A, Hunter T, Noel JP. The structure of the membrane distal phosphatase domain of RPTPalpha reveals interdomain flexibility and an SH2 domain interaction region. Biochemistry. 2003 Jul 8;42(26):7904-14. PMID:12834342 doi:http://dx.doi.org/10.1021/bi0340503

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