First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
1osl
From Proteopedia
| 1osl, 20 NMR models () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | LACI (Escherichia coli) | ||||||||
| Related: | 1l1m | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence
Interaction of regulatory DNA binding proteins with their target sites is usually preceded by binding to nonspecific DNA. This speeds up the search for the target site by several orders of magnitude. We report the solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA. The same set of residues can switch roles from a purely electrostatic interaction with the DNA backbone in the nonspecific complex to a highly specific binding mode with the base pairs of the cognate operator sequence. The protein-DNA interface of the nonspecific complex is flexible on biologically relevant time scales that may assist in the rapid and efficient finding of the target site.
Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes., Kalodimos CG, Biris N, Bonvin AM, Levandoski MM, Guennuegues M, Boelens R, Kaptein R, Science. 2004 Jul 16;305(5682):386-9. PMID:15256668
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1OSL is a 4 chains structure of sequences from Escherichia coli. Full experimental information is available from OCA.
Reference
- Kalodimos CG, Biris N, Bonvin AM, Levandoski MM, Guennuegues M, Boelens R, Kaptein R. Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes. Science. 2004 Jul 16;305(5682):386-9. PMID:15256668 doi:10.1126/science.1097064
Page seeded by OCA on Wed Oct 21 09:38:54 2009
