1o18

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1o18, resolution 70.00Å ()
Non-Standard Residues:
Related: 1m8q, 1mvw
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE

Publication Abstract from PubMed

Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), thought to represent the end of the power stroke. Unaveraged tomograms from a 25-nm longitudinal section of insect flight muscle preserved native structural variation. Recurring crossbridge motifs that repeat every 38.7 nm along the actin filament were extracted from the tomogram and classified by correspondence analysis into 25 class averages, which improved the signal to noise ratio. Models based on the atomic structures of actin and of myosin subfragment 1 were rebuilt to fit 11 class averages. A real-space refinement procedure was applied to quantitatively fit the reconstructions and to minimize steric clashes between domains introduced during the fitting. These combined procedures show that no single myosin head structure can fit all the in situ crossbridges. The validity of the approach is supported by agreement of these atomic models with fluorescent probe data from vertebrate muscle as well as with data from regulatory light chain crosslinking between heads of smooth muscle heavy meromyosin when bound to actin.

Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle., Chen LF, Winkler H, Reedy MK, Reedy MC, Taylor KA, J Struct Biol. 2002 Apr-May;138(1-2):92-104. PMID:12160705

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1o18 is a 30 chain structure with sequence from Gallus gallus and Oryctolagus cuniculus. Full crystallographic information is available from OCA.

See Also

Reference

  • Chen LF, Winkler H, Reedy MK, Reedy MC, Taylor KA. Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle. J Struct Biol. 2002 Apr-May;138(1-2):92-104. PMID:12160705

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