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1nxu
From Proteopedia
| 1nxu, resolution 1.80Å () | |||||||||
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| Non-Standard Residues: | |||||||||
| Gene: | YIAK OR B3575 (Escherichia coli) | ||||||||
| Domains: | Ldh_2 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
CRYSTAL STRUCTURE OF E. COLI HYPOTHETICAL OXIDOREDUCTASE YIAK NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER82.
Escherichia coli YiaK catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH. It belongs to a large family of oxidoreductases that is conserved in archaea, bacteria, and eukaryotes but shows no sequence homology to other proteins. We report here the crystal structures at up to 2.0-A resolution of YiaK alone and in complex with NAD-tartrate. YiaK has a new polypeptide backbone fold and a novel mode of recognizing the NAD cofactor. In addition, NAD is bound in an unusual conformation, at the interface of a dimer of the enzyme. The crystallographic analysis unexpectedly revealed the binding of tartrate in the active site. Enzyme kinetics studies confirm that tartrate and the related D-malate are inhibitors of YiaK. In contrast to most other enzymes where substrate binding produces a more closed conformation, the binding of NAD-tartrate to YiaK produces a more open active site. The free enzyme conformation is incompatible with NAD binding. His(44) is likely the catalytic residue of the enzyme.
A novel NAD-binding protein revealed by the crystal structure of 2,3-diketo-L-gulonate reductase (YiaK)., Forouhar F, Lee I, Benach J, Kulkarni K, Xiao R, Acton TB, Montelione GT, Tong L, J Biol Chem. 2004 Mar 26;279(13):13148-55. Epub 2004 Jan 12. PMID:14718529
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1NXU is a 2 chains structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A novel NAD-binding protein revealed by the crystal structure of 2,3-diketo-L-gulonate reductase (YiaK)., Forouhar F, Lee I, Benach J, Kulkarni K, Xiao R, Acton TB, Montelione GT, Tong L, J Biol Chem. 2004 Mar 26;279(13):13148-55. Epub 2004 Jan 12. PMID:14718529
Page seeded by OCA on Sun Nov 16 11:39:09 2008
Categories: Escherichia coli | Acton, T B. | Benach, J. | Forouhar, F. | Kulkarni, K. | Lee, I. | Montelione, G T. | NESG, Northeast Structural Genomics Consortium. | Rost, B. | Shastry, R. | Tong, L. | Xiao, R. | Hypothetical protein | Nesg | Northeast structural genomics consortium | Oxidoreductase | Protein structure initiative | Psi | Structural genomic
