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1n38

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1n38, resolution 2.80Å ()
Ligands: , ,
Gene: L1 (Mammalian orthoreovirus 3)
Domains: RdRP_5
Related: 1muk, 1mwh, 1n1h, 1n35
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed

Publication Abstract from PubMed

The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel.

RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:12464184

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1N38 is a Single protein structure of sequence from Mammalian orthoreovirus 3. Full crystallographic information is available from OCA.

Reference

RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:12464184

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