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1n34

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1n34, resolution 3.80Å ()
Sites: and
Ligands:
Related: 1j5e, 1fjg, 1ibl, 1ibk, 1ibm, 1n32, 1n33, 1n36
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Structure of the Thermus thermophilus 30S ribosomal subunit in the presence of codon and crystallographically disordered near-cognate transfer rna anticodon stem-loop mismatched at the first codon position

Publication Abstract from PubMed

A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity.

Selection of tRNA by the ribosome requires a transition from an open to a closed form., Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V, Cell. 2002 Nov 27;111(5):721-32. PMID:12464183

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1n34 is a 22 chain structure of Ribosomal protein S10, Ribosomal protein S11, Ribosomal protein S12, Ribosomal protein S13, Ribosomal protein S15, Ribosomal protein S17, Ribosomal protein S2, Ribosomal protein S20, Ribosomal protein S3, Ribosomal protein S4, Ribosomal protein THX and Ribosome with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

See Also

Reference

  • Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V. Selection of tRNA by the ribosome requires a transition from an open to a closed form. Cell. 2002 Nov 27;111(5):721-32. PMID:12464183

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