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1mz6
From Proteopedia
| 1mz6, resolution 2.90Å () | |||||||||
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| Ligands: | , | ||||||||
| Activity: | Exo-alpha-sialidase, with EC number 3.2.1.18 | ||||||||
| Domains: | Sialidase | ||||||||
| Related: | 1mz5, 1mr5, 1ms0, 1ms1, 1ms3, 1ms4, 1ms5, 1ms8, 1ms9 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Trypanosoma rangeli sialidase in complex with the inhibitor DANA
Publication Abstract from PubMed
The intracellular parasite Trypanosoma cruzi, the etiological agent of Chagas disease, sheds a developmentally regulated surface trans-sialidase, which is involved in key aspects of parasite-host cell interactions. Although it shares a common active site architecture with bacterial neuraminidases, the T. cruzi enzyme behaves as a highly efficient sialyltransferase. Here we report the crystal structure of the closely related Trypanosoma rangeli sialidase and its complex with inhibitor. The enzyme folds into two distinct domains: a catalytic beta-propeller fold tightly associated with a lectin-like domain. Comparison with the modeled structure of T. cruzi trans-sialidase and mutagenesis experiments allowed the identification of amino acid substitutions within the active site cleft that modulate sialyltransferase activity and suggest the presence of a distinct binding site for the acceptor carbohydrate. The structures of the Trypanosoma enzymes illustrate how a glycosidase scaffold can achieve efficient glycosyltransferase activity and provide a framework for structure-based drug design.
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1MZ6 is a 1 chain structure of sequence from Trypanosoma rangeli. Full crystallographic information is available from OCA.
Reference
- Buschiazzo A, Tavares GA, Campetella O, Spinelli S, Cremona ML, Paris G, Amaya MF, Frasch AC, Alzari PM. Structural basis of sialyltransferase activity in trypanosomal sialidases. EMBO J. 2000 Jan 4;19(1):16-24. PMID:10619840 doi:10.1093/emboj/19.1.16
Page seeded by OCA on Mon Feb 16 17:12:04 2009
