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1lbg
From Proteopedia
| 1lbg, resolution 4.80Å () | |||||||||
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| Gene: | LACI (Escherichia coli) | ||||||||
| Domains: | HTH_LACI, lacI | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY
The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene. The three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric operator DNA have been determined. These three structures show the conformation of the molecule in both the induced and repressed states and provide a framework for understanding a wealth of biochemical and genetic information. The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base pairs. The crystallographic structure of the complex with DNA suggests that the tetrameric repressor functions synergistically with catabolite gene activator protein (CAP) and participates in the quaternary formation of repression loops in which one tetrameric repressor interacts simultaneously with two sites on the genomic DNA.
Crystal structure of the lactose operon repressor and its complexes with DNA and inducer., Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P, Science. 1996 Mar 1;271(5253):1247-54. PMID:8638105
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1LBG is a 8 chains structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
- Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science. 1996 Mar 1;271(5253):1247-54. PMID:8638105
Page seeded by OCA on Tue Feb 17 06:16:24 2009
