First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
1jjx
From Proteopedia
| 1jjx, 20 NMR models () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Domains: | Lipocalin | ||||||||
| Related: | 1fdq | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein
Human brain-type fatty acid-binding protein (B-FABP) has been recombinantly expressed in Escherichia coli both unlabelled and 15N-enriched for structure investigation in solution using high-resolution NMR spectroscopy. The sequential assignments of the 1H and 15N resonances were achieved by applying multidimensional homo- and heteronuclear NMR experiments. The ensemble of the 20 final energy-minimized structures, representing human B-FABP in solution, have been calculated based on a total of 2490 meaningful distance constraints. The overall B-FABP structure exhibits the typical backbone conformation described for other members of the FABP family, consisting often antiparallel beta-strands (betaA to betaJ) that form two almost orthogonal beta-sheets, a helix-turn-helix motif that closes the beta-barrel on one side, and a short N-terminal helical loop. A comparison with the crystal structure of the same protein complexed with docosahexaenoic acid reveals only minor differences in both secondary structure and overall topology. Moreover, the NMR data indicate a close structural relationship between human B-FABP and heart-type FABP with respect to fatty acid binding inside the protein cavity.
Solution structure of fatty acid-binding protein from human brain., Rademacher M, Zimmerman AW, Ruterjans H, Veerkamp JH, Lucke C, Mol Cell Biochem. 2002 Oct;239(1-2):61-8. PMID:12479569
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1JJX is a 1 chain structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
- Rademacher M, Zimmerman AW, Ruterjans H, Veerkamp JH, Lucke C. Solution structure of fatty acid-binding protein from human brain. Mol Cell Biochem. 2002 Oct;239(1-2):61-8. PMID:12479569
Page seeded by OCA on Tue Feb 17 16:50:55 2009
