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1j59
From Proteopedia
| 1j59, resolution 2.50Å () | |||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
The crystallographic structure of the CAP-DNA complex at 3.0 A resolution has been reported previously. For technical reasons, the reported structure had been determined using a gapped DNA molecule lacking two phosphates important for CAP-DNA interaction. In this work, we report the crystallographic structure of the CAP-DNA complex at 2.5 A resolution using a DNA molecule having all phosphates important for CAP-DNA interaction. The present resolution permits unambiguous identification of amino acid-base and amino acid-phosphate hydrogen bonded contacts in the CAP-DNA complex. In addition, the present resolution permits accurate definition of the kinked DNA conformation in the CAP-DNA complex.
Structure of the CAP-DNA complex at 2.5 angstroms resolution: a complete picture of the protein-DNA interface., Parkinson G, Wilson C, Gunasekera A, Ebright YW, Ebright RE, Berman HM, J Mol Biol. 1996 Jul 19;260(3):395-408. PMID:8757802
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1J59 is a 6 chains structure with sequences from Escherichia coli. This structure supersedes the now removed PDB entry 1ber. The December 2003 RCSB PDB Molecule of the Month feature on Catabolite Activator Protein by David S. Goodsell is 10.2210/rcsb_pdb/mom_2003_12. Full crystallographic information is available from OCA.
Reference
- Parkinson G, Wilson C, Gunasekera A, Ebright YW, Ebright RE, Berman HM. Structure of the CAP-DNA complex at 2.5 angstroms resolution: a complete picture of the protein-DNA interface. J Mol Biol. 1996 Jul 19;260(3):395-408. PMID:8757802
Page seeded by OCA on Thu Jan 21 09:05:29 2010
