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1iz0
From Proteopedia
| 1iz0, resolution 2.30Å () | |||||
|---|---|---|---|---|---|
| Ligands: | |||||
| Non-Standard Residues: | |||||
| Activity: | NADPH:quinone reductase, with EC number 1.6.5.5 | ||||
| Domains: | ADH_zinc_N, Qor | ||||
| Related: | 1iyz | ||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||
| Coordinates: | save as pdb, mmCIF, xml | ||||
Crystal Structures of the Quinone Oxidoreductase from Thermus thermophilus HB8 and Its Complex with NADPH
The crystal structures of the zeta-crystalline-like soluble quinone oxidoreductase from Thermus thermophilus HB8 (QOR(Tt)) and of its complex with NADPH have been determined at 2.3- and 2.8-A resolutions, respectively. QOR(Tt) is composed of two domains, and its overall fold is similar to the folds of Escherichia coli quinone oxidoreductase (QOR(Ec)) and horse liver alcohol dehydrogenase. QOR(Tt) forms a homodimer in the crystal by interaction of the betaF-strands in domain II, forming a large beta-sheet that crosses the dimer interface. High thermostability of QOR(Tt) was evidenced by circular dichroic measurement. NADPH is located between the two domains in the QOR(Tt)-NADPH complex. The disordered segment involved in the coenzyme binding of apo-QOR(Tt) becomes ordered upon NADPH binding. The segment covers an NADPH-binding cleft and may serve as a lid. The 2'-phosphate group of the adenine of NADPH is surrounded by polar and positively charged residues in QOR(Tt), suggesting that QOR(Tt) binds NADPH more readily than NADH. The putative substrate-binding site of QOR(Tt), unlike that of QOR(Ec), is largely blocked by nearby residues, permitting access only to small substrates. This may explain why QOR(Tt) has weak p-benzoquinone reduction activity and is inactive with such large substrates of QOR(Ec) as 5-hydroxy-1,4-naphthoquinone and phenanthraquinone.
Crystal structures of the quinone oxidoreductase from Thermus thermophilus HB8 and its complex with NADPH: implication for NADPH and substrate recognition., Shimomura Y, Kakuta Y, Fukuyama K, J Bacteriol. 2003 Jul;185(14):4211-8. PMID:12837796
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1IZ0 is a 1 chain structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the quinone oxidoreductase from Thermus thermophilus HB8 and its complex with NADPH: implication for NADPH and substrate recognition., Shimomura Y, Kakuta Y, Fukuyama K, J Bacteriol. 2003 Jul;185(14):4211-8. PMID:12837796
Page seeded by OCA on Sun Nov 16 14:10:13 2008
