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1irl

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1irl, 1 NMR models ()
Domains: IL2
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



THE SOLUTION STRUCTURE OF THE F42A MUTANT OF HUMAN INTERLEUKIN 2

Publication Abstract from PubMed

Interleukin 2 (IL-2) is one of the major cytokines produced by T lymphocytes in response to antigen. It is a potent growth and differentiation factor for several cell-types and is structurally related to the four-helix bundle family of cytokines. Mutation of residue Phe42 to Ala abolishes binding to the alpha chain of the tri-partite IL-2 receptor. The three-dimensional structure of the F42A mutant IL-2 has been calculated by two dimensional NMR methods and compared to a structure of wild-type IL-2 determined by X-ray crystallography. The overall topology of the two structures is the same. The main differences between the structures are within the ill-defined loops connecting the helices and the region of the protein that is believed to interact with the alpha-chain of the receptor. Thus, the mutation of Phe42 to Ala does not perturb the overall three-dimensional structure of IL-2, and does not appear to change the putative binding sites for the beta and gamma chains of the receptor. The structural differences observed in this mutant suggest that the replacement of Phe42 with Ala causes the re-orientation of neighbouring side-chains that are also involved in binding the alpha-chain of the receptor.

The solution structure of the F42A mutant of human interleukin 2., Mott HR, Baines BS, Hall RM, Cooke RM, Driscoll PC, Weir MP, Campbell ID, J Mol Biol. 1995 Apr 14;247(5):979-94. PMID:7723044

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1IRL is a 1 chain structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

  • Mott HR, Baines BS, Hall RM, Cooke RM, Driscoll PC, Weir MP, Campbell ID. The solution structure of the F42A mutant of human interleukin 2. J Mol Biol. 1995 Apr 14;247(5):979-94. PMID:7723044 doi:10.1006/jmbi.1994.0194

Page seeded by OCA on Tue Feb 17 17:52:24 2009

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