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1io7
From Proteopedia
| 1io7, resolution 1.50Å () | |||||
|---|---|---|---|---|---|
| Ligands: | |||||
| Domains: | CypX | ||||
| Related: | 1io8, 1io9 | ||||
| |||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||
| Coordinates: | save as pdb, mmCIF, xml | ||||
THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES
Crystal structures of a thermostable cytochrome P450 (CYP119) and a site-directed mutant, (Phe24Leu), from the acidothermophilic archaea Sulfolobus solfataricus were determined at 1.5-2.0 A resolution. We identify important crystallographic waters in the ferric heme pocket, observe protein conformational changes upon inhibitor binding, and detect a unique distribution of surface charge not found in other P450s. An analysis of factors contributing to thermostability of CYP119 of these high resolution structures shows an apparent increase in clustering of aromatic residues and optimum stacking. The contribution of aromatic stacking was investigated further with the mutant crystal structure and differential scanning calorimetry.
Thermophilic cytochrome P450 (CYP119) from Sulfolobus solfataricus: high resolution structure and functional properties., Park SY, Yamane K, Adachi S, Shiro Y, Weiss KE, Maves SA, Sligar SG, J Inorg Biochem. 2002 Sep 20;91(4):491-501. PMID:12237217
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1IO7 is a 2 chains structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Thermophilic cytochrome P450 (CYP119) from Sulfolobus solfataricus: high resolution structure and functional properties., Park SY, Yamane K, Adachi S, Shiro Y, Weiss KE, Maves SA, Sligar SG, J Inorg Biochem. 2002 Sep 20;91(4):491-501. PMID:12237217
Page seeded by OCA on Sun Nov 16 18:32:03 2008
