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1hyt

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1hyt, resolution 1.70Å ()
Ligands: , , ,
Activity: Thermolysin, with EC number 3.4.24.27
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

RE-DETERMINATION AND REFINEMENT OF THE COMPLEX OF BENZYLSUCCINIC ACID WITH THERMOLYSIN AND ITS RELATION TO THE COMPLEX WITH CARBOXYPEPTIDASE A

Publication Abstract from PubMed

The complex of benzylsuccinic acid with thermolysin has been redetermined at 1.7-A resolution and refined to a crystallographic residual of 15.7%. In contrast to the prior study, which was to 2.3-A resolution, and without the benefit of refinement (Bolognesi, M. C. and Matthews, B. W. (1979) J. Biol. Chem. 254, 634-639), the present analysis shows that it is the D- rather than the L-isomer of benzylsuccinic acid that binds. The stereochemistry of the zinc-carboxylate interaction is now seen to be syn, as is also observed in all known zinc-carboxylate complexes of both thermolysin and carboxypeptidase A. The mode of binding of the beta-carboxylate resembles the presumed geometry of the tetrahedral transition state and, as such, is consistent with the commonly accepted mechanism of action of thermolysin and of carboxypeptidase A.

Redetermination and refinement of the complex of benzylsuccinic acid with thermolysin and its relation to the complex with carboxypeptidase A., Hausrath AC, Matthews BW, J Biol Chem. 1994 Jul 22;269(29):18839-42. PMID:8034637

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1hyt is a 1 chain structure with sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA.

See Also

Reference

  • Hausrath AC, Matthews BW. Redetermination and refinement of the complex of benzylsuccinic acid with thermolysin and its relation to the complex with carboxypeptidase A. J Biol Chem. 1994 Jul 22;269(29):18839-42. PMID:8034637

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