1hjk

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1hjk, resolution 2.30Å ()
Ligands: , ,
Non-Standard Residues:
Gene: PHOA (Escherichia coli)
Activity: Alkaline phosphatase, with EC number 3.1.3.1
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

ALKALINE PHOSPHATASE MUTANT H331Q

Publication Abstract from PubMed

Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.

Trapping and visualization of a covalent enzyme-phosphate intermediate., Murphy JE, Stec B, Ma L, Kantrowitz ER, Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1hjk is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA.

See Also

Reference

  • Murphy JE, Stec B, Ma L, Kantrowitz ER. Trapping and visualization of a covalent enzyme-phosphate intermediate. Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408

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