|1hcq, resolution 2.40Å ()|
THE CRYSTAL STRUCTURE OF THE ESTROGEN RECEPTOR DNA-BINDING DOMAIN BOUND TO DNA: HOW RECEPTORS DISCRIMINATE BETWEEN THEIR RESPONSE ELEMENTS
The nuclear hormone receptors are a superfamily of ligand-activated DNA-binding transcription factors. We have determined the crystal structure (at 2.4 A) of the fully specific complex between the DNA-binding domain from the estrogen receptor and DNA. The protein binds as a symmetrical dimer to its palindromic binding site consisting of two 6 bp consensus half sites with three intervening base pairs. This structure reveals how the protein recognizes its own half site sequence rather than that of the related glucocorticoid receptor, which differs by only two base pairs. Since all nuclear hormone receptors recognize one or the other of these two consensus half site sequences, this recognition mechanism applies generally to the whole receptor family.
The crystal structure of the estrogen receptor DNA-binding domain bound to DNA: how receptors discriminate between their response elements., Schwabe JW, Chapman L, Finch JT, Rhodes D, Cell. 1993 Nov 5;75(3):567-78. PMID:8221895
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1hcq is a 8 chain structure with sequence from Homo sapiens. The September 2003 RCSB PDB Molecule of the Month feature on Estrogen Receptor by David S. Goodsell is 10.2210/rcsb_pdb/mom_2003_9. Full crystallographic information is available from OCA.
- Schwabe JW, Chapman L, Finch JT, Rhodes D. The crystal structure of the estrogen receptor DNA-binding domain bound to DNA: how receptors discriminate between their response elements. Cell. 1993 Nov 5;75(3):567-78. PMID:8221895
- Wang LH, Yang XY, Zhang X, Mihalic K, Fan YX, Xiao W, Howard OM, Appella E, Maynard AT, Farrar WL. Suppression of breast cancer by chemical modulation of vulnerable zinc fingers in estrogen receptor. Nat Med. 2004 Jan;10(1):40-7. Epub 2003 Dec 14. PMID:14702633 doi:10.1038/nm969
- Hicks JM, Hsu VL. The extended left-handed helix: a simple nucleic acid-binding motif. Proteins. 2004 May 1;55(2):330-8. PMID:15048824 doi:10.1002/prot.10630