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1gnu
From Proteopedia
| 1gnu, resolution 1.75Å () | |||||||||
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| Domains: | GABARAP | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
GABA(A) RECEPTOR ASSOCIATED PROTEIN GABARAP
The gamma-aminobutyric acid receptor type A (GABA(A)) receptor-associated protein (GABARAP) has been reported to mediate the interaction between the GABA(A) receptor and microtubules. We present the three-dimensional structure of GABARAP obtained by x-ray diffraction at 1.75 A resolution. The structure was determined by molecular replacement using the structure of the homologous protein GATE-16. NMR spectroscopy of isotope-labeled GABARAP showed the structure in solution to be compatible with the overall fold but showed evidence of conformation heterogeneity that is not apparent in the crystal structure. We assessed the binding of GABARAP to peptides derived from reported binding partner proteins, including the M3-M4 loop of the gamma2 subunit of the GABA(A) receptor and the acidic carboxyl-terminal tails of human alpha- and beta-tubulin. There is a small area of concentrated positive charge on one surface of GABARAP, which we found interacts weakly with all peptides tested, but we found no evidence for specific binding to the proposed physiological target peptides. These results are compatible with a more general role in membrane targeting and transportation for the GABARAP family of proteins.
The X-ray crystal structure and putative ligand-derived peptide binding properties of gamma-aminobutyric acid receptor type A receptor-associated protein., Knight D, Harris R, McAlister MS, Phelan JP, Geddes S, Moss SJ, Driscoll PC, Keep NH, J Biol Chem. 2002 Feb 15;277(7):5556-61. Epub 2001 Nov 29. PMID:11729197
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1GNU is a 1 chain structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Knight D, Harris R, McAlister MS, Phelan JP, Geddes S, Moss SJ, Driscoll PC, Keep NH. The X-ray crystal structure and putative ligand-derived peptide binding properties of gamma-aminobutyric acid receptor type A receptor-associated protein. J Biol Chem. 2002 Feb 15;277(7):5556-61. Epub 2001 Nov 29. PMID:11729197 doi:10.1074/jbc.M109753200
Page seeded by OCA on Tue Feb 17 15:27:57 2009
