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1gdt
From Proteopedia
| 1gdt, resolution 3.00Å () | |||||||||
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| Gene: | TNPR (Escherichia coli) | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE
The structure of gamma delta resolvase complexed with a 34 bp substrate DNA has been determined at 3.0 A resolution. The DNA is sharply bent by 60 degrees toward the major groove and away from the resolvase catalytic domains at the recombination crossover point. The C-terminal one third of resolvase, which was disordered in the absence of DNA, forms an arm and a 3-helix DNA-binding domain on the opposite side of the DNA from the N-terminal domain. The arms wrap around the minor groove of the central 16 bp, and the DNA-binding domains interact with the major grooves near the outer boundaries of the binding site. The resolvase dimer is asymmetric, particularly in the arm region, implying a conformational adaptability that may be important for resolvase binding to different DNA sites in the synaptosome. It also raises the possibility of a sequential single-strand cleavage mechanism.
Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site., Yang W, Steitz TA, Cell. 1995 Jul 28;82(2):193-207. PMID:7628011
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1gdt is a 6 chain structure of Resolvase with sequence from Escherichia coli. Full crystallographic information is available from OCA.
See Also
Reference
- Yang W, Steitz TA. Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site. Cell. 1995 Jul 28;82(2):193-207. PMID:7628011
