1fjg

From Proteopedia

Jump to: navigation, search
1fjg, resolution 3.00Å ()
Ligands: , , , ,
Related: 1qd7, 1fjf
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN

Publication Abstract from PubMed

The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process.

Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics., Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V, Nature. 2000 Sep 21;407(6802):340-8. PMID:11014183

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1fjg is a 22 chain structure with sequence from Thermus thermophilus. The October 2000 RCSB PDB Molecule of the Month feature on Ribosome by David S. Goodsell is 10.2210/rcsb_pdb/mom_2000_10. Full crystallographic information is available from OCA.

See Also

Reference

  • Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V. Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics. Nature. 2000 Sep 21;407(6802):340-8. PMID:11014183 doi:10.1038/35030019
  • Shortle D. Composites of local structure propensities: evidence for local encoding of long-range structure. Protein Sci. 2002 Jan;11(1):18-26. PMID:11742118

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools