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1ff1
From Proteopedia
| 1ff1, 20 NMR models () | |||||||||
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| Ligands: | |||||||||
| Domains: | EH, EH | ||||||||
| Related: | 1eh2, 1f8h | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
STRUCTURE OF THE SECOND EPS15 HOMOLOGY DOMAIN OF HUMAN EPS15 IN COMPLEX WITH PTGSSSTNPFL
Eps15 homology (EH) domains are protein interaction modules that recognize Asn-Pro-Phe (NPF) motifs in their biological ligands to mediate critical events during endocytosis and signal transduction. To elucidate the structural basis of the EH-NPF interaction, the solution structures of two EH-NPF complexes were solved using NMR spectroscopy. The first complex contains a peptide representing the Hrb C-terminal NPFL motif; the second contains a peptide in which an Arg residue substitutes the C-terminal Leu. The NPF residues are almost completely embedded in a hydrophobic pocket on the EH domain surface and the backbone of NPFX adopts a conformation reminiscent of the Asx-Pro type I beta-turn motif. The residue directly following NPF is crucial for recognition and is required to complete the beta-turn. Five amino acids on the EH surface mediate specific recognition of this residue through hydrophobic and electrostatic contacts. The complexes explain the selectivity of the second EH domain of Eps15 for NPF over DPF motifs and reveal a critical aromatic interaction that provides a conserved anchor for the recognition of FW, WW, SWG and HTF ligands by other EH domains.
Molecular mechanism of NPF recognition by EH domains., de Beer T, Hoofnagle AN, Enmon JL, Bowers RC, Yamabhai M, Kay BK, Overduin M, Nat Struct Biol. 2000 Nov;7(11):1018-22. PMID:11062555
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1FF1 is a 2 chains structure of sequences from Homo sapiens. Full experimental information is available from OCA.
Reference
- de Beer T, Hoofnagle AN, Enmon JL, Bowers RC, Yamabhai M, Kay BK, Overduin M. Molecular mechanism of NPF recognition by EH domains. Nat Struct Biol. 2000 Nov;7(11):1018-22. PMID:11062555 doi:10.1038/80924
Page seeded by OCA on Wed Feb 18 10:00:06 2009
Categories: Homo sapiens | Beer, T De. | Bowers, R C. | Enmon, J L. | Hoofnagle, A N. | Kay, B K. | Overduin, M. | Yamabhai, M. | Calcium binding | Complex | Ef-hand | Eh domain | Hrb | Npf | Signaling domain
