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|1efn, resolution 2.50Å ()|
|Gene:||HIV-1 NEF (Homo sapiens), HIV-1 NEF (Human immunodeficiency virus 1)|
HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN
The crystal structure of the conserved core of HIV-1 Nef has been determined in complex with the SH3 domain of a mutant Fyn tyrosine kinase (a single amino acid substitution, Arg-96 to isoleucine), to which Nef binds tightly. The conserved PxxP sequence motif of Nef, known to be important for optimal viral replication, is part of a polyproline type II helix that engages the SH3 domain in a manner resembling closely the interaction of isolated peptides with SH3 domains. The Nef-SH3 structure also reveals how high affinity and specificity in the SH3 interaction is achieved by the presentation of the PxxP motif within the context of the folded structure of Nef.
Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain., Lee CH, Saksela K, Mirza UA, Chait BT, Kuriyan J, Cell. 1996 Jun 14;85(6):931-42. PMID:8681387
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Lee CH, Saksela K, Mirza UA, Chait BT, Kuriyan J. Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain. Cell. 1996 Jun 14;85(6):931-42. PMID:8681387
- Arold S, Hoh F, Domergue S, Birck C, Delsuc MA, Jullien M, Dumas C. Characterization and molecular basis of the oligomeric structure of HIV-1 nef protein. Protein Sci. 2000 Jun;9(6):1137-48. PMID:10892807 doi:10.1110/ps.9.6.1137
- Clout NJ, Basak A, Wieligmann K, Bateman OA, Jaenicke R, Slingsby C. The N-terminal domain of betaB2-crystallin resembles the putative ancestral homodimer. J Mol Biol. 2000 Dec 1;304(3):253-7. PMID:11090271 doi:10.1006/jmbi.2000.4197
- Larson SM, Davidson AR. The identification of conserved interactions within the SH3 domain by alignment of sequences and structures. Protein Sci. 2000 Nov;9(11):2170-80. PMID:11152127 doi:10.1110/ps.9.11.2170