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1e07

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1e07 ()
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Model of human carcinoembryonic antigen by homology modelling and curve-fitting to experimental solution scattering data

Publication Abstract from PubMed

MFE23 is a single chain Fv antibody that has a high affinity for carcinoembryonic antigen (CEA). A full homology model for CEA based on V-type, I-type and C2-type immunoglobulin folds, 28 oligosaccharides and the interdomain angle of CD2 was validated using solution scattering data. The superimposition of the intermolecular contacts observed in our recent crystal structure of MFE23 with the N-terminal domain of CEA permitted the MFE23-CEA complex to be modelled. Good surface and electrostatic complementarity and carbohydrate-unhindered access of MFE23 with the indentation between the first two CEA domains was observed. The model is supported by biochemical data and provides insight on the high affinity of MFE23 for CEA.

Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23., Boehm MK, Perkins SJ, FEBS Lett. 2000 Jun 9;475(1):11-6. PMID:10854848

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1e07 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

  • Boehm MK, Perkins SJ. Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23. FEBS Lett. 2000 Jun 9;475(1):11-6. PMID:10854848
  • Boehm MK, Corper AL, Wan T, Sohi MK, Sutton BJ, Thornton JD, Keep PA, Chester KA, Begent RH, Perkins SJ. Crystal structure of the anti-(carcinoembryonic antigen) single-chain Fv antibody MFE-23 and a model for antigen binding based on intermolecular contacts. Biochem J. 2000 Mar 1;346 Pt 2:519-28. PMID:10677374
  • Boehm MK, Mayans MO, Thornton JD, Begent RH, Keep PA, Perkins SJ. Extended glycoprotein structure of the seven domains in human carcinoembryonic antigen by X-ray and neutron solution scattering and an automated curve fitting procedure: implications for cellular adhesion. J Mol Biol. 1996 Jun 21;259(4):718-36. PMID:8683578

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