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1dva
From Proteopedia
| 1dva, resolution 3.00Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||||
| Non-Standard Residues: | , , | ||||||||
| Activity: | Coagulation factor VIIa, with EC number 3.4.21.21 | ||||||||
| Domains: | Tryp_SPc, EGF_CA | ||||||||
| Related: | 1dan, 1cvw, 1qfk, 1fak | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN THE PEPTIDE EXOSITE INHIBITOR E-76 AND COAGULATION FACTOR VIIA
Potent anticoagulants have been derived by targeting the tissue factor-factor VIIa complex with naive peptide libraries displayed on M13 phage. The peptides specifically block the activation of factor X with a median inhibitory concentration of 1 nM and selectively inhibit tissue-factor-dependent clotting. The peptides do not bind to the active site of factor VIIa; rather, they work by binding to an exosite on the factor VIIa protease domain, and non-competitively inhibit activation of factor X and amidolytic activity. One such peptide (E-76) has a well defined structure in solution determined by NMR spectroscopy that is similar to the X-ray crystal structure when complexed with factor VIIa. These structural and functional studies indicate an allosteric 'switch' mechanism of inhibition involving an activation loop of factor VIIa and represent a new framework for developing inhibitors of serine proteases.
Peptide exosite inhibitors of factor VIIa as anticoagulants., Dennis MS, Eigenbrot C, Skelton NJ, Ultsch MH, Santell L, Dwyer MA, O'Connell MP, Lazarus RA, Nature. 2000 Mar 30;404(6777):465-70. PMID:10761907
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1DVA is a 8 chains structure with sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Dennis MS, Eigenbrot C, Skelton NJ, Ultsch MH, Santell L, Dwyer MA, O'Connell MP, Lazarus RA. Peptide exosite inhibitors of factor VIIa as anticoagulants. Nature. 2000 Mar 30;404(6777):465-70. PMID:10761907 doi:10.1038/35006574
Page seeded by OCA on Mon Mar 22 14:49:50 2010
