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1dl6
From Proteopedia
| 1dl6, 25 NMR models () | |||||||||
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| Ligands: | |||||||||
| Domains: | TFIIB_Zn_Ribbon | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
SOLUTION STRUCTURE OF HUMAN TFIIB N-TERMINAL DOMAIN
Transcription factor IIB (TFIIB) is an essential component in the formation of the transcription initiation complex in eucaryal and archaeal transcription. TFIIB interacts with a promoter complex containing the TATA-binding protein (TBP) to facilitate interaction with RNA polymerase II (RNA pol II) and the associated transcription factor IIF (TFIIF). TFIIB contains a zinc-binding motif near the N-terminus that is directly involved in the interaction with RNA pol II/TFIIF and plays a crucial role in selecting the transcription initiation site. The solution structure of the N-terminal residues 2-59 of human TFIIB was determined by multidimensional NMR spectroscopy. The structure consists of a nearly tetrahedral Zn(Cys)3(His)1 site confined by type I and "rubredoxin" turns, three antiparallel beta-strands, and disordered loops. The structure is similar to the reported zinc-ribbon motifs in several transcription-related proteins from archaea and eucarya, including Pyrococcus furiosus transcription factor B (PfTFB), human and yeast transcription factor IIS (TFIIS), and Thermococcus celer RNA polymerase II subunit M (TcRPOM). The zinc-ribbon structure of TFIIB, in conjunction with the biochemical analyses, suggests that residues on the beta-sheet are involved in the interaction with RNA pol II/TFIIF, while the zinc-binding site may increase the stability of the beta-sheet.
Structure of a (Cys3His) zinc ribbon, a ubiquitous motif in archaeal and eucaryal transcription., Chen HT, Legault P, Glushka J, Omichinski JG, Scott RA, Protein Sci. 2000 Sep;9(9):1743-52. PMID:11045620
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1DL6 is a 1 chain structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
- Chen HT, Legault P, Glushka J, Omichinski JG, Scott RA. Structure of a (Cys3His) zinc ribbon, a ubiquitous motif in archaeal and eucaryal transcription. Protein Sci. 2000 Sep;9(9):1743-52. PMID:11045620
Page seeded by OCA on Mon Feb 16 10:00:35 2009
