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1d8k
From Proteopedia
| 1d8k, 1 NMR models () | |||||||||
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| Domains: | TFIIE_beta | ||||||||
| Related: | 1d8j | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
SOLUTION STRUCTURE OF THE CENTRAL CORE DOMAIN OF TFIIE BETA
Human general transcription factor TFIIE consists of two subunits, TFIIEalpha and TFIIEbeta. Recently, TFIIEbeta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. Here, the central core domain of human TFIIEbeta (TFIIEbetac) has been identified by a limited proteolysis. This solution structure has been determined by NMR. It consists of three helices with a beta hairpin at the C-terminus, resembling the winged helix proteins. However, TFIIEbetac shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow. A model will be proposed that TFIIE stabilizes the preinitiation complex by binding not only to the general transcription factors together with RNA polymerase II but also to the promoter DNA, where double-stranded DNA starts to open to be single-stranded upon activation of the preinitiation complex.
Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface., Okuda M, Watanabe Y, Okamura H, Hanaoka F, Ohkuma Y, Nishimura Y, EMBO J. 2000 Mar 15;19(6):1346-56. PMID:10716934
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1D8K is a 1 chain structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
- Okuda M, Watanabe Y, Okamura H, Hanaoka F, Ohkuma Y, Nishimura Y. Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface. EMBO J. 2000 Mar 15;19(6):1346-56. PMID:10716934
Page seeded by OCA on Mon Feb 16 14:55:31 2009
