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|1d3c, resolution 1.78Å ()|
|Related:||1cdg, 1cxi, 1cxf, 1cxk, 1cxl, 2dij|
MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN
The enzyme cyclodextrin glycosyltransferase is closely related to alpha-amylases but has the unique ability to produce cyclodextrins (circular alpha(1-->4)-linked glucoses) from starch. To characterize this specificity we determined a 1.8-A structure of an E257Q/D229N mutant cyclodextrin glycosyltransferase in complex with its product gamma-cyclodextrin, which reveals for the first time how cyclodextrin is competently bound. Across subsites -2, -1, and +1, the cyclodextrin ring binds in a twisted mode similar to linear sugars, giving rise to deformation of its circular symmetry. At subsites -3 and +2, the cyclodextrin binds in a manner different from linear sugars. Sequence comparisons and site-directed mutagenesis experiments support the conclusion that subsites -3 and +2 confer the cyclization activity in addition to subsite -6 and Tyr-195. On this basis, a role of the individual residues during the cyclization reaction cycle is proposed.
The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution., Uitdehaag JC, Kalk KH, van Der Veen BA, Dijkhuizen L, Dijkstra BW, J Biol Chem. 1999 Dec 3;274(49):34868-76. PMID:10574960
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Uitdehaag JC, Kalk KH, van Der Veen BA, Dijkhuizen L, Dijkstra BW. The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution. J Biol Chem. 1999 Dec 3;274(49):34868-76. PMID:10574960