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1ci4
From Proteopedia
| 1ci4, resolution 1.90Å () | |||||||||||
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| Non-Standard Residues: | |||||||||||
| Domains: | BAF | ||||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||
THE CRYSTAL STRUCTURE OF HUMAN BARRIER-TO-AUTOINTEGRATION FACTOR (BAF)
Barrier-to-autointegration factor (BAF) is a host cell protein that plays a crucial role in retroviral integration. Preintegration complexes (PICs) stripped of BAF lose their normal integration activity, which can be restored by incubation with purified BAF. BAF bridges double-stranded DNA both intra- and intermolecularly in a non-sequence-specific manner, leading to the formation of a nucleoprotein network. BAF also binds to the nuclear protein lamina-associated polypeptide 2 (LAP2), and is localized with chromatin during interphase and mitosis. The crystal structure of homodimeric human BAF has been determined to 1.9 A resolution. The fold of the BAF monomer resembles that of the second domain of RuvA. This comparison revealed the presence of the helix-hairpin-helix (HhH) nonspecific DNA binding motif within BAF. A novel feature of BAF's HhH motif is the occupation of the metal binding site by the epsilon-amino group of Lys 6, providing an alternative means of sequestering positive charge. Mutational analysis corroborates the HhH motif's prominent role in DNA binding and argues against a previously proposed helix-turn-helix (HTH) binding site located in another region of the monomer. A model of BAF bridging DNA via the HhH motif is proposed.
Structural basis of DNA bridging by barrier-to-autointegration factor., Umland TC, Wei SQ, Craigie R, Davies DR, Biochemistry. 2000 Aug 8;39(31):9130-8. PMID:10924106
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1CI4 is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Umland TC, Wei SQ, Craigie R, Davies DR. Structural basis of DNA bridging by barrier-to-autointegration factor. Biochemistry. 2000 Aug 8;39(31):9130-8. PMID:10924106
Page seeded by OCA on Tue Feb 17 06:03:14 2009
