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1cfi
From Proteopedia
| 1cfi, 17 NMR models () | |||||
|---|---|---|---|---|---|
| Non-Standard Residues: | |||||
| Activity: | Coagulation factor IXa, with EC number 3.4.21.22 | ||||
| Domains: | GLA | ||||
| |||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||
| Coordinates: | save as pdb, mmCIF, xml | ||||
NMR STRUCTURE OF CALCIUM ION-BOUND GAMMA-CARBOXY-GLUTAMIC ACID-RICH DOMAIN OF FACTOR IX
We have determined the Ca(II)-bound structure of factor IX, residues 1-47, by nuclear magnetic resonance (NMR) spectroscopy. The amino-terminal 47 residues include the gamma-carboxyglutamic acid-rich and aromatic amino acid stack domains, and this region is responsible for Ca(II)-dependent phospholipid binding in factor IX. Protons in the 1-47 amino acid sequence were assigned using standard two-dimensional homonuclear NMR experiments. A total of 851 distance restraints and 57 torsion angle restraints were used to generate 17 final structures by distance geometry and simulated annealing methods. The backbone RMSD to the geometric average is 0.6 +/- 0.1 A. The Ca(II)-bound structure is substantially more ordered with increased helical content compared to the apo-factor IX (1-47) structure. The global fold is similar to the crystal structure of the Ca(II)-bound Gla domain of prothrombin fragment I from residues 12 to 47 (RMSD approximately 1.3 A), but the backbone conformation differs in the first 11 residues, particularly between residues 3 and 6. The amino-terminal nine Gla residues are oriented to the interior of the protein and suggest an internal Ca(II) binding pocket. The carboxyl-terminal three Gla residues are exposed to solvent. The majority of hydrophobic residues are required to stabilize a globular core in the carboxyl-terminal three-quarters of the molecule. However, a hydrophobic surface patch in the amino-terminal region may represent a phospholipid binding site in factor IX.
Structure of the calcium ion-bound gamma-carboxyglutamic acid-rich domain of factor IX., Freedman SJ, Furie BC, Furie B, Baleja JD, Biochemistry. 1995 Sep 26;34(38):12126-37. PMID:7547952
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1CFI is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
Structure of the calcium ion-bound gamma-carboxyglutamic acid-rich domain of factor IX., Freedman SJ, Furie BC, Furie B, Baleja JD, Biochemistry. 1995 Sep 26;34(38):12126-37. PMID:7547952
Page seeded by OCA on Mon Jun 30 20:40:54 2008
