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1c3w
From Proteopedia
| 1c3w, resolution 1.55Å () | |||||||||
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| Sites: | , , , , , , , , , , , , , and | ||||||||
| Ligands: | , , | ||||||||
| Related: | 1brx | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
BACTERIORHODOPSIN/LIPID COMPLEX AT 1.55 A RESOLUTION
Th?e atomic structure of the light-driven ion pump bacteriorhodopsin and the surrounding lipid matrix was determined by X-ray diffraction of crystals grown in cubic lipid phase. In the extracellular region, an extensive three-dimensional hydrogen-bonded network of protein residues and seven water molecules leads from the buried retinal Schiff base and the proton acceptor Asp85 to the membrane surface. Near Lys216 where the retinal binds, transmembrane helix G contains a pi-bulge that causes a non-proline? kink. The bulge is stabilized by hydrogen-bonding of the main-chain carbonyl groups of Ala215 and Lys216 with two buried water molecules located between the Schiff base and the proton donor Asp96 in the cytoplasmic region. The results indicate extensive involvement of bound water molecules in both the structure and the function of this seven-helical membrane protein. A bilayer of 18 tightly bound lipid chains forms an annulus around the protein in the crystal. Contacts between the trimers in the membrane plane are mediated almost exclusively by lipids.
Structure of bacteriorhodopsin at 1.55 A resolution., Luecke H, Schobert B, Richter HT, Cartailler JP, Lanyi JK, J Mol Biol. 1999 Aug 27;291(4):899-911. PMID:10452895
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1C3W is a 1 chain structure with sequence from Halobacterium salinarum. The March 2002 RCSB PDB Molecule of the Month feature on Bacteriorhodopsin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2002_3. Full crystallographic information is available from OCA.
Reference
- Luecke H, Schobert B, Richter HT, Cartailler JP, Lanyi JK. Structure of bacteriorhodopsin at 1.55 A resolution. J Mol Biol. 1999 Aug 27;291(4):899-911. PMID:10452895 doi:10.1006/jmbi.1999.3027
Page seeded by OCA on Wed Jan 20 22:33:56 2010
