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|1bbt, resolution 2.60Å ()|
METHODS USED IN THE STRUCTURE DETERMINATION OF FOOT AND MOUTH DISEASE VIRUS
The structure of foot-and-mouth disease virus (FMDV) strain O1 BFS 1860 has been determined to 2.9 A resolution using the molecular-replacement method [Acharya, Fry, Stuart, Fox, Rowlands & Brown (1989). Nature (London), 337, 709-716]. Crystals of the virus with average dimensions 0.12 x 0.06 x 0.12 mm belong to space group I23, a = 345 A with 1/12 of the icosahedral particle per asymmetric unit giving fivefold noncrystallographic redundancy. Oscillation diffraction photographs were collected at the SERC Synchrotron Radiation Source at Daresbury in accordance with strict disease security regulations. The ambiguity in particle orientation was resolved using a self-rotation function and starting estimates of the phases to 8 A were derived from the known structures of two picornaviruses similarly oriented in the I23 unit cell. The phases were refined and extended using iterative averaging and solvent flattening with the implementation of a simple automatic envelope-determination procedure to increase the phasing power available.
Methods used in the structure determination of foot-and-mouth disease virus., Fry E, Acharya R, Stuart D, Acta Crystallogr A. 1993 Jan 1;49 ( Pt 1):45-55. PMID:8382928
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1bbt is a 4 chain structure with sequence from Foot-and-mouth disease virus. The August 2001 RCSB PDB Molecule of the Month feature on Poliovirus and Rhinovirus by David S. Goodsell is 10.2210/rcsb_pdb/mom_2001_8. Full crystallographic information is available from OCA.
- Fry E, Acharya R, Stuart D. Methods used in the structure determination of foot-and-mouth disease virus. Acta Crystallogr A. 1993 Jan 1;49 ( Pt 1):45-55. PMID:8382928