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1b2y
From Proteopedia
| 1b2y, resolution 3.20Å () | |||||||||
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| Sites: | , , , , , and | ||||||||
| Ligands: | , , | ||||||||
| Non-Standard Residues: | , , , | ||||||||
| Activity: | Alpha-amylase, with EC number 3.2.1.1 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
STRUCTURE OF HUMAN PANCREATIC ALPHA-AMYLASE IN COMPLEX WITH THE CARBOHYDRATE INHIBITOR ACARBOSE
Crystal structures of human pancreatic alpha-amylase (HPA) in complex with naturally occurring inhibitors have been solved. The tetrasaccharide acarbose and a pseudo-pentasaccharide of the trestatin family produced identical continuous electron densities corresponding to a pentasaccharide species, spanning the -3 to +2 subsites of the enzyme, presumably resulting from transglycosylation. Binding of the acarviosine core linked to a glucose residue at subsites -1 to +2 appears to be a critical part of the interaction process between alpha-amylases and trestatin-derived inhibitors. Two crystal forms, obtained at different values of pH, for the complex of HPA with the protein inhibitor from Phaseolus vulgaris (alpha-amylase inhibitor) have been solved. The flexible loop typical of the mammalian alpha-amylases was shown to exist in two different conformations, suggesting that loop closure is pH-sensitive. Structural information is provided for the important inhibitor residue, Arg-74, which has not been observed previously in structural analyses.
Crystal structures of human pancreatic alpha-amylase in complex with carbohydrate and proteinaceous inhibitors., Nahoum V, Roux G, Anton V, Rouge P, Puigserver A, Bischoff H, Henrissat B, Payan F, Biochem J. 2000 Feb 15;346 Pt 1:201-8. PMID:10657258
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1B2Y is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Nahoum V, Roux G, Anton V, Rouge P, Puigserver A, Bischoff H, Henrissat B, Payan F. Crystal structures of human pancreatic alpha-amylase in complex with carbohydrate and proteinaceous inhibitors. Biochem J. 2000 Feb 15;346 Pt 1:201-8. PMID:10657258
Page seeded by OCA on Mon Mar 22 14:47:56 2010
