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2ack
From Proteopedia
| 2ack, resolution 2.40Å () | |||||||||
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| Sites: | and | ||||||||
| Ligands: | |||||||||
| Activity: | Acetylcholinesterase, with EC number 3.1.1.7 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
ACETYLCHOLINESTERASE COMPLEXED WITH EDROPHONIUM, MONOCHROMATIC DATA
See also AChE inhibitors and substrates (Part II).
Acetylcholinesterase (AChE) is one of nature's fastest enzymes, despite the fact that its three-dimensional structure reveals its active site to be deeply sequestered within the molecule. This raises questions with respect to traffic of substrate to, and products from, the active site, which may be investigated by time-resolved crystallography. In order to address one aspect of the feasibility of performing time-resolved studies on AChE, a data set has been collected using the Laue technique on a trigonal crystal of Torpedo californica AChE soaked with the reversible inhibitor edrophonium, using a total X-ray exposure time of 24 ms. Electron-density maps obtained from the Laue data, which are of surprisingly good quality compared with similar maps from monochromatic data, show essentially the same features. They clearly reveal the bound ligand, as well as a structural change in the conformation of the active-site Ser200 induced upon binding.
Static Laue diffraction studies on acetylcholinesterase., Ravelli RB, Raves ML, Ren Z, Bourgeois D, Roth M, Kroon J, Silman I, Sussman JL, Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1359-66. PMID:10089512
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Edrophonium (EDR) is stacked between the aromatic rings of , near the TcAChE which consists of S200, E327, and H440.
About this Structure
2ACK is a Single protein structure of sequence from Torpedo californica. This structure supersedes the now removed PDB entry 1ack. Full crystallographic information is available from OCA.
Additional Resources
For additional information, see: Alzheimer's Disease
Reference
Static Laue diffraction studies on acetylcholinesterase., Ravelli RB, Raves ML, Ren Z, Bourgeois D, Roth M, Kroon J, Silman I, Sussman JL, Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1359-66. PMID:10089512 Page seeded by OCA on Sat May 3 18:52:49 2008
