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11gs
From Proteopedia
| 11gs, resolution 2.30Å () | |||||||||
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| Sites: | , , , , and | ||||||||
| Ligands: | , , | ||||||||
| Gene: | GSTP1 (Homo sapiens) | ||||||||
| Activity: | Glutathione transferase, with EC number 2.5.1.18 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Glutathione s-transferase complexed with ethacrynic acid-glutathione conjugate (form ii)
The diuretic drug ethacrynic acid, an inhibitor of pi class glutathione S-transferase, has been tested in clinical trials as an adjuvant in chemotherapy. We recently solved the crystal structure of this enzyme in complex with ethacrynic acid and its glutathione conjugate. Here we present a new structure of the ethacrynic-glutathione conjugate complex. In this structure the ethacrynic moiety of the complex is shown to bind in a completely different orientation to that previously observed. Thus there are at least two binding modes possible, an observation of great importance to the design of second generation inhibitors of the enzyme.
The glutathione conjugate of ethacrynic acid can bind to human pi class glutathione transferase P1-1 in two different modes., Oakley AJ, Lo Bello M, Mazzetti AP, Federici G, Parker MW, FEBS Lett. 1997 Dec 8;419(1):32-6. PMID:9426214
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
11gs is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Oakley AJ, Lo Bello M, Mazzetti AP, Federici G, Parker MW. The glutathione conjugate of ethacrynic acid can bind to human pi class glutathione transferase P1-1 in two different modes. FEBS Lett. 1997 Dec 8;419(1):32-6. PMID:9426214
